By C B Anfinsen
ADVANCES IN PROTEIN CHEMISTRY VOL 3.
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In bicarbonate bufFer, the synthesis with PU is inhibited, rather than augmented, by added dicarboxylata, presumably through competitive inhibition of enzymes concerned with intermediary tranaformationu of dicarboxylic aubstrates. The time relatiom of AS and AL formation from PU and NH: are much the aame in experiments with added fumrate in phosphate butler 88 without dicarboxyllic acid in bicarbonate butler (121). Phoephate is a neceeaary component of the reaction s p h m (122). With phosphatefree bicarbonatwrsline there ie no amino wid eyntheak, or a very smsll one, unless catalytic amounta of dicarboxylic acid are added.
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